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HPLC Method Development And Validation

Course summary
3 days
Professional Training

Course description

HPLC Method Development And Validation

HPLC is a potent analytical tool, allowing for the separation, identification and quantification of drug substances. It also presents many challenges, from the correct use of instrumentation, to analysing results, to the fulfilment of the regulatory requirements of method validation.

Over two days, this course will offer delegates the opportunity to examine the core aspects of HPLC theory and method development, including troubleshooting. Over the course of an optional third day, delegates will examine quantitation and method validation.

Suitability - Who should attend?

This course will be beneficial to personnel working in the following areas:

  • Analytical (scientist/chemist)
  • Analytical development
  • Validation 
  • Technician
  • Project Manager
  • Pharmacist
  • Formulation
  • R&D
  • Stability 
  • CMC

Outcome / Qualification etc.

HPLC Method Development And Validation

Training Course Content

  • Know the important chemical parameters affecting HPLC separation
  • Be familiar with chromatographic theory
  • Understand HPLC retention mechanisms and stationary phase types
  • Know how to improve resolution between closely-eluting peaks
  • Be able to troubleshoot HPLC system faults
  • Approach the development and validation of quantitative HPLC methods in a logical, scientifically-sound manner

Why choose PTI

On average, delegates of their online academies said: 

Quality of content:


85% had applied content during the course to their role

Delivery rating:


About provider

PTI - Pharmaceutical Training International

PTI - Pharmaceutical Training International - Training for the Pharma Industry

PTI is the global leader in professional education for the pharmaceutical industry, delivering a broad course portfolio that covers the industry spectrum: R&D, Clinical Development, Generics, Bio-pharmaceuticals, fine Chemicals, Agro-chemicals, Medical Devices, Animal Health, Manufacturing and Regulations. PTI's open courses,...

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Average rating 5

Based on 2 reviews

Product Development Specialists, PharOS Ltd.
Very interactive course and it was oriented for our specific needs for improvement! Really enjoyed the ability to interact and discuss with the trainer the problems and issues I am facing everyday and queries about analytical challenges.
Laboratory Analyst, Surepharm Services Ltd.
The course covers a range of levels to keep each course member on the same level of understanding. I enjoyed discussion options for improvements that can be applied. Especially enjoyed the troubleshooting and validation sections!
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Course rating
Based on 2 reviews
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